Effects Of Ppar-y-/- In Inflammatory Parameter Of Liver The Rats Submitted To High-fat Diet And Aerobic Physical Exercise

Peroxisome proliferator-activated receptor gamma (PPAR) are nuclear receptors belonging to the steroid receptor superfamily, the PPAR-y interact with proteins that acts as co-activators, and their main role is lipid mobilization, glucose metabolism, morphogenesis, and inflammatory response. The regular practice of exercise is suggested as an important agent in anti-inflammatory responses, combat of cardiovascular diseases, type II diabetes and metabolic diseases. The aim of this study were analyze the effects of aerobic physical exercise associated to high-fat diet in histological and inflammatory parameters in liver of PPAR-y rats. The mices were underwent into 4 groups (n=8): wild type and high-fat diet (WT-HF); knockout high-fat diet (KO-HF); wild type, high-fat diet and trained (WT-HFT); knockout trained (KO-HFT). The animals were adapted to a treadmill (10min in 10 m/min). This study were approved by CEUA (Protocol 112/13). The initial body weight did not present significance difference, by the way, the WT-HF group increased the final body weight (37,21±1,71) compared to WT-HFT (29,35±1,71). Similarly, the aerobic exercise reduced the weight gain in WT-HFT group (8,83±1,67) in relation to WT-HF (16,15±1,67). In the same perspective, the trained group (WT-HFT) reduced the absolute liver weight (1,16±0,37) than WT-HF (1,53±0,37), the relative liver weight did not present significative differences. The HF increased the fat index (%) in KO-HF group (9,23±0,94) where aerobic exercise reduced this percentage in KO-HFT group (5,10±0,94). In histological analyzes, the periodic acid-reactive Schiff (PAS) did not present significative differences, however, the KO-HF group reduced the massonMMVs trichrome (7,07±0,67) compared to WT-HF (11,10±0,67), the WT-HF animals enlarged the collagen (11,10±0,67) in relation to trained animals WT-HFT (7,29±0,67). The IL-6 increased in KO-HF group (18,68±3,93) compared to KO-HFT (14,74±3,93). The NE-KB were most expressed in the KO-HF group (16,90±2,68) than KO-HFT (14,21±2,68) and less marked than WT-HF group (20,55±0,72). Already WT-HFT reduced the NF-KB stain (13,28±0,66) compared to WT-HF (20,55±0,66). Nevertheless, the aerobic physical exercise repaired the collagen and connective tissue in the trained animals, reduced the inflammatory markers where HF leverages this obesogenic environment. Agencia de Fomento: Coordenagao de Aperfeicoamento de Pessoal de Nivel Superior-Brasil (CAPES)-Finance Code-001. 

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