Resumo

Ashwagandha is a popular Ayurvedic herb for treating numerous diseases and also increasing human health. Enhanced cardiorespiratory endurance can enhance human physiological, metabolic, and functional abilities. Aswagandha root has extensive role for the enhancement of sports performance such as Ashwagandha root extract improves cardiorespiratory endurance and quality of life in healthy athletic individuals without producing any adverse effect. Main aim of this experimental study was to find out the bioactive components present in the aswagandha root powder and test overall in-vitro antioxidant ability of aswagandha root powder. Methods: For the preparation of sample aswagandha root was collected from local market and dried in microwave in different watt (360watt, 540watt and 900watt). The dried sample was grounded in mixer grinder and then it was taken for the preparation of extraction by using methanol and water. For doing HPLC (High Performance Liquid Chromatography), HPLC graded methanol and water were used. The extracted samples were taken for the analysis of total polyphenolic compound (TPC), total flavonoid compound (TFC) and free radical scavenging capacity was determined by 2,2-diphenyl 1-picrylhydrazyl radical (DPPH) method. Ferric reducing antioxidant power (FRAP) was done to measure to analyse the reducing power of the extract. For the qualitative and quantitative identification of bioactive compounds HPLC was done. Overall moisture, protein, fat and carbohydrate and ash content of aswagandha root were determined by using moisture analyser, kjeldhal method, Soxhlet method, anthrone method and maple furnace respectively.

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