Effects Of Rats Exhaustive Swimming On Atp Synthesis Activity Of Kydney Mitochondria

Introduction
In order to study the mechanisms of exercise-induced exhaustion and porteinuria, the experiment was designed to
investigate exhaustive exercise-induced oxidative stress on the functions of kidney mitochondria.

Methods
30 male Sprague-Dawley rats were randomly divided into four groups（control group, n=7; immediate postexercise
group, n=7; 1h postexercise group, n=8 and 24h postexercise group , n=8）, and the changes were measured that the
content of MDA、ATP syntheses activity and total calcium in the kidney mitochondria in the different phases were
observed.

Results
The results are as follows: when compared with control group , the MDA content significantly rises immediately after
exercise（p<0.05）and it is the same to 1h and 24h postexercise group（p<0.01）;The ATP synthesis activity of
kidney mitochondria of immediate postexercise, 1h postexercise group individually declines extremely as compared
with control group（p<0.01）while it does not recover to a normal value 24h after exercise; The mitochondria total
calcium of 1h and 24h groups is markedly higher than the control group （p<0.01; p<0.05）.

Conclusions
The findings suggest that exhaustive swimming can lead to an increase of mitochondria lipid peroxidation , a decrease
of ATP synthesis activity of kidney mitochondria and irregularity of the steady state of the mitochondria calcium while
the mitochondria calcium accumulation probably results in the uncoupling of intramitochondria oxidative
phosphorylation and anormality of kidney functions. It might be one of the important reasons of exercise-induced
exhaustion and porteinuria.

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