Experimental Study On Treatment Of Athletic Menstrual Cycle Irregularities With Tradional Chinese Medicione

Introduction
Athletic Menstrual Cycle Irregularities (AMI) is a special disease of female athletes after the training. Statistics data had
showed that the incidence of AMI in female athletes was 30% to 35% (very few reached 86%). AMI can cause achylic
anemia, osteoporotic fracture, and infertility in female athletes, which will affect seriously their level of training and
sports and even mental health. The theory of modern sports medicine is that AMI will be treated with heteropathy,
reducing training intensity, and even stopping training. However, good results have not been obtained yet and some
side-effect appear. In the study, we tested chiefly the curative effect and mechanism of traditional Chinese medicine on
AMI.

Methods
Three-month-old female SD rats (n=48,body wt 220±3g,non-mating) were randomly assigned to A, B, C three groups
(16 each): A, control group; B, swimming group; C, traditional Chinese medicine group. The AMI model was prepared
by swimming exhausted exercise. At the same time, the rats from the traditional Chinese medicine group were
subjected to perfusion with the traditional Chinese medicine for 21 days. After 21 days, blood samples were obtained
from rats femoral arteries and centrifugated to obtain serum for Estradiol ( E2) and
Hydroxyproline（HP）concentration measurements. The right hemispheres of rat brains for electron microscopy were
rapidly excised and fixed in 3.5% glutaraldehyde. Transmission electron microscope was used to observe the changes of
the ultrastructure of blood capillary and nervous tissue in cerebral cortices; In addition, the ovary from each group were
weighed , then viscera coefficients were calculated. Data were analyzed with statistical method.

Results
Compared with B group, the swell of neuroglial cell surrounding the capillary of telencephalon in the C group and the
swell of mitochondria had been obviously improved, and the dimensions of vacuoles had obviously decreased under
electron microscope. The weights of ovaries had increased (P<0.05), the level of serum Estradiol (E2) had obviously
increased (P<0.01), and the level of serum Hydroxyproline (HP) obviously decreased (P<0.01).

Discussion
The results showed that the level of serum Estradiol (E2) and the weight of the ovary obviously decreased in the
Athletic Menstrual Cycle Irregularities. The weight coefficient of the ovary and the level of serum Estradiol (E2) raised
obviously (P<0.05) by the traditional Chinese medicine, therefore, it indicated that the traditional Chinese medicine
could effectively regulated the Athletic Menstrual Cycle by both increasing the level of serum Estradiol (E2) and
promoting the follicular growth. Due to the decreasing of the level of HP, it suggested that the traditional Chinese
medicine had some good effects on osteoporosis by both improving decompensation and preventing osseous losses, and
had a hygienical effect on osseous trauma resulting from sports. The results from transmission electron microscope in
telencephalon indicated that the traditional Chinese medicine had promoted recover from fatigue and eliminated the
fatigue of central nervous system. It is expected that this traditional Chinese medicine could promote the sports ability
of female athletes by increasing the level of serum Estradiol (E2), effectively regulating the AMI, and promoting the
release of growth hormone.

References
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