Titre du document / Document title

Detection in urine of 4-methyl-2-hexaneamine, a doping agent

Auteur(s) / Author(s)

PERRENOUD Laurent (1) ; SAUGY Martial (1) ; SAUDAN Christophe (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Swiss Anti Doping Laboratory, University Center of Legal Medicine, Geneva and Lausanne, Chemin des Croisettes 22, 1066 Epalinges, SUISSE

Résumé / Abstract

Stimulants are banned in-competition for all categories of sports by the World Anti-Doping Agency. A simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay employing electrospray ionisation in positive mode was developed in that work for the quantification in urine specimens of 4-methyl-2-hexaneamine, a primary amine exhibiting sympathomimetic properties. Following a simple pretreatment procedure, the analyte was separated using a gradient mobile phase on reverse phase C8 column. Selected reaction monitoring m/z 116.2→57.3 was specific for detection of 4-methyl-2-hexaneamine and the assay exhibited a linear dynamic range of 50-700ng/mL. The validated method has been successfully applied to analyze the target compound in food supplements as well as in urine specimens. The administered drug (40 mg) was detected at the level of 350 ng/mL in the urine up to 4 days.

Revue / Journal Title

Journal of chromatography. B   ISSN 1570-0232 

Source / Source

2009, vol. 877, no29, pp. 3767-3770 [4 page(s) (article)] (12 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (2002) (Revue)

Mots-clés anglais / English Keywords

Gas chromatography ; Liquid chromatography ; Mass spectrometry ; Stimulant ; Doping ; Biological fluid ; Urine ; Detection ;

Mots-clés français / French Keywords

Chromatographie phase gazeuse ; Chromatographie phase liquide ; Spectrométrie masse ; Stimulant ; Dopage ; Liquide biologique ; Urine ; Détection ;

Mots-clés espagnols / Spanish Keywords

Cromatografía fase gaseosa ; Cromatografía fase líquida ; Espectrometría masa ; Estimulante ; Doping ; Líquido biológico ; Orina ; Detección ;

Mots-clés d’auteur / Author Keywords

Doping ; Stimulant ; Mass spectrometry ; Liquid chromatography ; Gas chromatography ;

Localisation / Location

INIST-CNRS, Cote INIST : 8577 B, 35400017012296.0450

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