Artigo novo em folha, com inumeras referencias clicaveis : gratuito : Boa Leitura !
Gene Doping and Sport
Theodore Friedmann,1,* Olivier Rabin,2 Mark S. Frankel3,
1 University of California at San Diego, La Jolla, CA 92093, USA.
2 World Anti-Doping Agency (WADA), Montreal, Quebec H4Z 1B7, Canada.
3 American Association for the Advancement of Science, Washington, DC 20005, USA.
* T.F. is chair of WADA’s Gene Doping Expert Group and has performed research sponsored by WADA.
Author for correspondence. E-mail: mfrankel@aaas.org
We humans have long sought to enhance ourselves beyond normal through cosmetic surgery and drugs. Science is increasingly becoming humanity’s partner and handmaiden in those efforts (1) and has added genetic manipulation to our enhancement tool kit. Many forms of human enhancement are becoming more feasible, sought-after, and even justifiable in the quest for healthier, happier, and longer lives.
Around the world, people have been exposed to the notion of human enhancement through sport, as some athletes seek a boost to success, stardom, and financial reward. In the past, doping and cheating in sport have been enabled by advances in pharmacology and physiology. Recently, the successful development of gene therapy has provided the concepts, tools, opportunity, and, for some, justification for genetic modification of functions that affect normal human traits, including athletic performance. This intersection of science and sport raises fundamental ethical and policy issues that neither domain can resolve absent a broader societal conversation (2). As science progresses and sport and antidoping authorities express increasing concerns, the time is right to look at how advances in genetics are affecting sport in ways unexpected just a decade ago.
Genetic Manipulation for Doping
Some early experimental studies illustrate the potential of gene therapy for treating diseases (3–8). Although mostgene therapy approaches involve gain-of-function expression of exogenous transgenes, other methods for genetic modification have also emerged (9–15). A definitive approach to genetic modification for therapy would involve an emerging technology of site-specific sequence correction of disease-causing mutations, as through the use of zinc finger–associated recombinational methods (16).
Although highly effective in some models, these gene therapy techniques are imperfect and still highly risky, as demonstrated by severe adverse events such as treatment-induced leukemia, or even deaths (17–19). Nevertheless, it is inevitable that, as the science and techniques mature, these same methods and concepts will be applied to broader nontherapeutic uses, including gene-based "enhancement" of human traits linked to sport.
|
Toward that end, genetic methods have been used, for instance, to demonstrate enhanced muscle function from the insulin-like growth factor (IGF-1) or follistatin transgenes (20, 21) and stably increased, regulatable, erythropoietin-enhanced blood production in primates (22). One of the most widely discussed transcriptional modulation approaches has involved small molecule modulators of peroxisomal proliferator-activated receptor delta (PPAR-
![{delta}](http://www.sciencemag.org/math/delta.gif)
![{delta}](http://www.sciencemag.org/math/delta.gif)
![{delta}](http://www.sciencemag.org/math/delta.gif)
Not surprisingly, these scientific approaches are known in sport communities and are coming temptingly close to human doping. A German athletic coach was found attempting to obtain Repoxygen, a gene-transfer vector that induces expression of the erythropoietin gene (24). A Chinese genetics laboratory reportedly offered gene-based manipulations before the 2008 Olympic Games in Beijing (25). It is not clear whether these or other similar attemptsreached the stage of actual use in human athletes, but there seem to be few technical barriers standing in the way.
Genetic Tools for Doping Detection
Traditional approaches to detection of doping in sport have been based on chemical or molecular detection of the doping agent or of markers reflecting the physiological or metabolic effect(s) of the agent (e.g., chemical assays for steroids and stimulants, molecular identification of foreign erythropoietin, and detection of abnormally high erythrocyte production following exogenous erythropoietin exposure). Although this is the most direct approach, new assays are constantly needed to respond to chemical modifications that make some drugs more difficult to detect, and therefore more prone to doping abuse.
A potentially more powerful detection method has emerged, based on the concept that chemical, biological, or genetic doping agents are likely to produce broad metabolic, genetic, and proteomic changes. These changes are now detectable by techniques such as microarray- or sequence-based transcriptional profiling and proteomic and metabolomic analyses that can define molecular "signatures" of exposure to specific doping agents, or families of drugs, or methods. Such signatures may be used to identify perturbed physiological systems, even in the absence of knowledge of, or assays for, specific doping agents. This approach is similar to that commonly used in searches for molecular signatures of oncogenesis, developmental disorders, and so on (26, 27).
Highly concerned by the risk of gene doping, the World Anti-Doping Agency (WADA) (28), which has retained gene doping in its list of prohibited substances and methods since 2004, has sponsored international research teams with early results providing growing credence to the utility of molecular signatures in doping detection. For instance, exposure of murine myoblasts to IGF-1 has been shown to induce transcriptional and proteomic changes that mayeventually constitute a "signature" specific for exogenous IGF-1 exposure (29, 30). Of course, the application of these kinds of global assays would require rigorous validation of a connection with specific doping agents or methods.
Marketing Gene Doping
The challenges posed to sports organizations concerned with gene doping are compounded by the ubiquity of the Internet, relatively unconstrained by geographical boundaries, which, when fueled with private commercial interests, creates a powerful marketing tool for promotion and distribution of performance-enhancing agents. An industry has emerged to cater to the desire of athletes and their coaches to find a competitive edge.
Athletes are an especially vulnerable population in the marketing of performance enhancement (31). Reputable athletes or coaches with little knowledge of genetics are at a disadvantage in assessing "scientific" claims that appear in advertisements. Marketing is particularly worrisome when the science is still a work in progress, when a person’s health can be adversely affected, and when consumer knowledge about genetics is low. Althoughadvertisements promoting products that promise to enhance athletic performance have pervaded the Internet for many years, recently it has become home for advertisements that promote products to "alter muscle genes...by activating your genetic machinery" (32), or that state "your genetic limitations are a thing of the past!" (33) or "Finally, every bodybuilder can be genetically gifted!" (34).
Conclusion
The stakes are high in competitive sport. Enter the science of genetics and the increasing ability to modify genes for medical and performance enhancement purposes. As a result, the former chairman of WADA proclaimed that "You would have to be blind not to see that the next generation of doping will be genetic" (35). As others have observed, "What is clear ... is just how impatient some coaches and athletes are to find new and ingenious ways to cheat. First it was steroids, then EPO [erythropoietin], then human growth hormone—and now the illicit grail seems to be gene therapy" (36). The global marketplace is ready to meet the demand in ways that will inevitably include untested, and perhaps unregulated, products and exaggerated claims. Although commercial Web sites may be "biased, and unreliable by rigorous scientific standards, they are a principal source of information for many athletes and should be monitored when looking for evidence of developing trends in doping" (37).
Accompanying those developments is the emergence of a community that alleges shortcomings in the testing process and blames the antidoping effort for stimulating "an arms race between regulators and the cheats" (38). Others question why certain enhancement technologies are banned while others remain legal and argue that athletes should be free to use virtually any enhancing agents that science makes available to them (39). Scientists are not mere bystanders in these matters. The 2010 Winter Olympic Games and other major sport events present goodopportunities for researchers to reaffirm their responsibilities to conduct and report their work by means consistent with international ethics codes of clinical research. They also must be aware that some athletes and coaches will be tempted, prematurely and unwisely, to take advantage of results packaged by some as performance enhancement "breakthroughs," even if they are untested in humans and the only "breakthrough" is faster or stronger mice (40, 41).
References and Notes
- 1. E. A. Williams, Good, Better, Best: The Human Quest for Enhancement, Washington, DC, 1 and 2 June 2006 (Workshop summary report, AAAS, Washington, DC, 2007);www.aaas.org/spp/sfrl/projects/human_enhancement/pdfs/HESummaryReport.pdf.
- 2. There have long been divergent views of sport doping and the value of antidoping regulation (41). Some commentators have addressed how the use of performance-enhancing drugs in sports and their regulation have adversely affected fair competition (42). Others have suggested that drug- or gene-based doping causes little or no harm to athletes, sport, and public policy and that the controversies surrounding doping could readily be obviated by simply carrying out doping under medical supervision (43).
- 3. D. B. Kohn, F. Candotti, Gene therapy fulfilling its promise. N. Engl. J. Med. 360, 518 (2009).[Free Full Text]
- 4. S. Hacein-Bey-Abina et al., Sustained correction of X-linked severe combined immunodeficiency by ex vivo gene therapy. N. Engl. J. Med. 346, 1185 (2002).[Abstract/Free Full Text]
- 5. A. Aiuti et al., Gene therapy for immunodeficiency due to adenosine deaminase deficiency. N. Engl. J. Med. 360, 447 (2009).[Abstract/Free Full Text]
- 6. A. M. Maguire et al., Safety and efficacy of gene transfer for Leber’s congenital amaurosis. N. Engl. J. Med. 358, 2240 (2008).[Abstract/Free Full Text]
- 7. N. Cartier et al., Hematopoietic stem cell gene therapy with a lentiviral vector in X-linked adrenoleukodystrophy.Science 326, 818 (2009).[Abstract/Free Full Text]
- 8. M. K. Brenner, Developing T-cell therapies for cancer in an academic setting. Adv. Exp. Med. Biol. 610, 88 (2008).[CrossRef] [Web of Science] [Medline]
- 9. E. R. Barton-Davis, L. Cordier, D. I. Shoturma, S. E. Leland, H. L. Sweeney, Aminoglycoside antibiotics restore dystrophin function to skeletal muscles of mdx mice. J. Clin. Invest. 104, 375 (1999).[Web of Science] [Medline]
- 10. A. Aartsma-Rus et al., Exploring the frontiers of therapeutic exon skipping for Duchenne muscular dystrophy by double targeting within one or multiple exons. Mol. Ther. 14, 401 (2006). [CrossRef] [Web of Science] [Medline]
- 11. G. McClorey, H. M. Moulton, P. L. Iversen, S. Fletcher, S. D. Wilton, Antisense oligonucleotide-induced exon skipping restores dystrophin expression in vitro in a canine model of DMD. Gene Ther. 13, 1373 (2006).[CrossRef] [Web of Science] [Medline]
- 12. E. Abranches et al., Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis in the embryo. PLoS ONE 4, e6286 (2009). [CrossRef] [Medline]
- 13. J. Alper, Geron gets green light for human trial of ES cell-derived product. Nat. Biotechnol. 27, 213 (2009).[Web of Science] [Medline]
- 14. U. Dressel et al., The peroxisome proliferator-activated receptor beta/delta agonist, GW501516, regulates the expression of genes involved in lipid catabolism and energy uncoupling in skeletal muscle cells. Mol. Endocrinol.17, 2477 (2003).[Abstract/Free Full Text]
- 15. D. Grimm, M. A. Kay, Therapeutic application of RNAi: Is mRNA targeting finally ready for prime time? Clin. Investig. 117, 3633 (2007). [CrossRef]
- 16. D. Carroll, Progress and prospects: zinc-finger nucleases as gene therapy agents. Gene Ther. 15, 1463 (2008).[CrossRef] [Web of Science] [Medline]
- 17. S. Hacein-Bey-Abina et al., A serious adverse event after successful gene therapy for X-linked severe combined immunodeficiency. N. Engl. J. Med. 348, 255 (2003).[Free Full Text]
- 18. B. C. Engel et al., Prolonged pancytopenia in a gene therapy patient with ADA-deficient SCID and trisomy 8 mosaicism: A case report. Blood 109, 503 (2007).[Abstract/Free Full Text]
- 19. A. J. Thrasher et al., Gene therapy: X-SCID transgene leukaemogenicity. Nature 443, E5 (2006). [Medline]
- 20. S. Lee, E. R. Barton, H. L. Sweeney, R. P. Farrar, Viral expression of insulin-like growth factor-I enhances muscle hypertrophy in resistance-trained rats. J. Appl. Physiol. 96, 1097 (2004).[Abstract/Free Full Text]
- 21. J. Kota et al., Sci. Transl. Med. 1, 6ra15 (2009).[Abstract/Free Full Text]
- 22. V. M. Rivera et al., Long-term pharmacologically regulated expression of erythropoietin in primates following AAV-mediated gene transfer. Blood 105, 1424 (2005).[Abstract/Free Full Text]
- 23. V. A. Narkar et al., AMPK and PPARdelta agonists are exercise mimetics. Cell 134, 405 (2008).[CrossRef] [Web of Science] [Medline]
- 24. DW-World, Deutsche-Welle, www.dw-world.de/dw/article/0,2144,1890782,00.html
- 25. Associated Press, 23 July 2009, www.msnbc.msn.com/id/25816605/.
- 26. F. J. Rauscher, 3rd, The year ahead. Cancer Res. 69, 1 (2009).[Free Full Text]
- 27. A. Anguiano et al., Gene expression profiles of tumor biology provide a novel approach to prognosis and may guide the selection of therapeutic targets in multiple myeloma. J. Clin. Oncol. 27, 4197 (2009).[Abstract/Free Full Text]
- 28. Established in 1999, WADA has been the principal agency responsible for antidoping activities in sport. Responsive to initiatives and requests from various stakeholders, WADA establishes global antidoping rules following extensive consultation processes with international sports federations, and governments of the world. More than 600 international sport organizations, including all 33 Olympic federations, have demonstrated their strong support of antidoping policies by adopting the World Anti-Doping Code and the related International Standards. Many active and former elite athletes are members of the consultative commissions or take part in the work of the executive boards of international sports federations, and the final rules and guidelines issued by WADA reflect both their input and support.
- 29. C. R. Bhasker, T. Friedmann, Insulin-like growth factor-1 coordinately induces the expression of fatty acid and cholesterol biosynthetic genes in murine C2C12 myoblasts. BMC Genomics 9, 535 (2008). [CrossRef] [Medline]
- 30. C. C. King, K. Bouic, T. Friedmann, A fractionation method to identify qauntitative changes in protein expression mediated by IGF-1 on the proteome of murine C2C12 myoblasts. Proteome Sci. 7, 28 (2009).[CrossRef] [Medline]
- 31. J. M. Tokish, M. S. Kocher, R. J. Hawkins, Ergogenic aids: A review of basic science, performance, side effects, and status in sports. Am. J. Sports Med. 32, 1543 (2004).[Abstract/Free Full Text]
- 32. Body Building.com, www.bodybuilding.com/store/vpx/no.html.
- 33. Netneutri.com, www.netnutri.com/browse.cfm/4,2954.html?aff=sh_z&gdftrk=gd.
- 34. Full Potential Training and Nutrition, www.fptnutrition.com/framework.cfm?indView=60_0.
- 35. O. Slot, Times Online (UK), 2 February 2006; www.timesonline.co.uk/tol/sport/article724765.ece.
- 36. G. Reynolds, New York Times, 3 June 2007;www.nytimes.com/2007/06/03/sports/playmagazine/0603play-hot.html.
- 37. M. N. Fedoruk, J. L. Rupert, Myostatin inhibition: a potential performance enhancement strategy? Scand. J. Med. Sci. Sports 18, 123 (2008). [Web of Science] [Medline]
- 38. Editor, Nature 454, 667 (2008). [Medline]
- 39. A. Miah, The Washington Post, 3 August 2008;www.washingtonpost.com/wp-dyn/content/article/2008/08/01/AR2008080103060.html.
- 40. M. Wenner, Sci. Am. Online (15 August 2008);www.scientificamerican.com/article.cfm?id=olympics-gene-doping-expert.
- 41. J. M. Hoberman, Mortal Engines: The Science of Performance and the Dehumanization of Sport (Free Press, NY, 1992).
- 42. D. H. Catlin, T. H. J. Murray, Performance-enhancing drugs, fair competition, and Olympic sport. JAMA 276, 231 (1996).[Abstract/Free Full Text]
- 43. B. Kayser, A. Mauron, A. Miah, Current anti-doping policy: A critical appraisal. BMC Med. Ethics 8, 2 (2007).[CrossRef] [Medline]
- 44. The authors thank the Greenwall Foundation for its support of this work through a grant to AAAS.
Comentários
Nenhum comentário realizado até o momentoPara comentar, é necessário ser cadastrado no CEV fazer parte dessa comunidade.